Journal: Genes & Development
Article Title: Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
doi: 10.1101/gad.1968110
Figure Lengend Snippet: Transposons, piRNAs, and piRNA precursors in zucchini and squash mutant ovaries. (A) Transcripts of gypsy, idefix, and ZAM transposons were detected by qPCR. (B) Individual piRNAs targeting gypsy and idefix were detected by qPCR. (C) Piwi protein levels in mutant and heterozygous ovary extracts were measured by Western blotting. Tubulin serves as loading control. Celera sequencing strain (S-strain) is shown in addition. (D) Three ∼100-nt regions of flamenco that are normally highly processed into piRNAs were detected by qPCR. The positions of these segments are indicated in Figure 4. qPCR data were normalized to internal controls rp49 (A,C) or bantam (B). Fold changes relative to heterozygous siblings are shown on a linear scale. Error bars represent one standard deviation over three technical replicates.
Article Snippet: Celera sequencing strain (S-strain) is shown in addition. ( D ) Three ∼100-nt regions of flamenco that are normally highly processed into piRNAs were detected by qPCR.
Techniques: Mutagenesis, Western Blot, Sequencing, Standard Deviation